ON THE ACCURACY, PRECISION AND STATISTICAL SIGNIFICANCE OF COMPRESSED RATIOS IN ISOBARIC LABELLING

Despite the outstanding advantages that isobaric labelling has to offer in quantitative proteomics, its precision and accuracy are undermined by ratio compression, which is consequence of the co-isolation of peptides within a selected isolation window. Here, using the two-proteomes approach we investigated the relationships between precision and accuracy on the statistical significance of peptide and protein ratios in three types of mass spectrometers.