Data and analysis script for "Nectar and floral morphology differ in evolutionary potential in novel pollination environments"

Datasets and code used in the manuscript "Nectar and floral morphology differ in evolutionary potential in novel pollination environments" accepted in New Phytologist.Data comprises flower morphology and nectar measurements on <i>Digitalis purpurea</i> in a common-garden experiment designed with a paternal half-sib full-sib nested crossing design.Phenotypic plasticity, heritability and evolvability of the different traits were estimated and compared between traits and types of traits.Analysis was carried out on RStudio and the scripts are coded and scripts are fully annotated with Rmarkdown (provided as .RMD files). Datasets provided in Microsoft Excel Comma Separated Values (.csv) files. An output of the MCMCglmm model is provided as RDATA file to be loaded to the R environment as the script to produce it may take several minutes to run and produce slightly different outputs due to the MCMC algorithm.<br><b>Abstract</b>· Plants can evolve rapidly after pollinator changes, but the response of different floral traits to novel selection can vary. Floral morphology is often expected to show high integration to maintain pollination accuracy, while nectar traits can be more environmentally sensitive. The relative role of genetic correlations and phenotypic plasticity in floral evolution remains unclear, particularly for nectar traits, and can be studied in the context of recent pollinator changes.· <i>Digitalis purpurea</i> shows rapid recent evolution of corolla morphology but not nectar traits following a range expansion with hummingbirds added as pollinators. We use this species to compare phenotypic plasticity, heritability, evolvability, and integration of floral morphology and nectar in a common garden.· Morphological traits showed higher heritability than nectar traits, and the proximal section of the corolla, which regulates access to nectar and underwent rapid change in introduced populations, presented lower integration than the rest of the floral phenotype. Nectar was more plastic than morphology, driven by highly plastic sugar concentration. Nectar production rate showed high potential to respond to selection.· These results explain the differential rapid evolution of floral traits previously observed in this species and show how intrafloral modularity determines variable evolutionary potential in morphological and nectar traits.