Evidence table, in chronological order from 2014 to 2021.

ZIKV = Zika virus, USUV = Usutu virus, VC = vector competence, VD = Virus/RNA detection in field collected mosquitoes, IT = Intrathoracic inoculations (examining upstream barriers to transmission), dpi = days post infection (includes manuscripts reporting dpe = days post exposure), RT = Room temperature. Infection Rate (IR, [#inf./#tested]) is defined as the percentage of mosquitoes containing virus in bodies or midguts (number positive/number tested). Terminology used for dissemination and transmission are expressed as cumulative (C) or stepwise (S) rates depending on the experimental design. For dissemination, we use cumulative dissemination rate (CDR, [#dissem./#tested]) or stepwise dissemination rate (SDR, [#dissem./#inf]), defined as the percentage of mosquitoes containing virus in head, legs+wings, or salivary glands/ovaries (number positive/number of engorged mosquitoes tested for infection [CDR] or number positive /number of infected mosquitoes [SDR]). For transmission, we use cumulative transmission rate (CTR, [#transm./#tested]) or stepwise transmission rate (STR, [#transm./#dissem.]), defined as the number of mosquitoes with virus in saliva or transmitting ZIKV to a mouse (number positive/number of engorged mosquitoes tested [CTR] or number positive/number disseminated infections [STR]). For authors that emphasized salivary gland testing, CSGR = cumulative salivary gland positivity rate is used. For the quality assessment TS = Total Score, MS = Methodology Score, QAS = percentile in quantile analysis among all articles scored.