CELSeq2-profiles for siRNA screening and daily timecourse for early reprogramming to pluripotency.

AIM: To find molecular signatures associated to the siRNA-mediated knockdowns in order to be able to identify similarities among different knockdowns. DESCRIPTION: Each sample includes biological triplicates for 35 siRNA-mediated knockdowns targeting 30 chromatin-associated proteins during in early reprogramming to iPS at day 6. A daily timecourse from reprogramming cells, without treatment from MEFs until day 6 is also included in triplicate. RNA was harvested for all samples in bulk and the CELSeq2 method was used to prepare the RNAseq libraries