BLASTx analysis of assembled insect SRA accessions - part 2

To identify insect species potentially associated with Ophiocordyceps-related yeast-like symbionts, transcriptomic SRA reads were aligned against the StYLS coding sequences of 18 ribosomal proteins and cytochrome c oxidase subunit I (COI). Read mapping was performed with Magic‑BLAST v1.7.0. Libraries with ≥20 aligned reads were downloaded. Reads were then subjected to quality control using the BBTools suite and assembled with SPAdes v4.2.0 in “meta” mode using default parameters. The resulting transcripts were queried against the NCBI core-nr database using DIAMOND BLASTx to identify fungal transcripts belonging to genera within the family Ophiocordycipitaceae. The output files from the BLASTx analysis of 43 of the 143 assembled libraries are provided (please see 10.5281/zenodo.18504810 for the other 100 files). Sequence similarity searches were performed using a customized tabular output (-outfmt 6) with the NCBI search metrics reported in this order: qseqid qlen sseqid stitle slen pident length mismatch gapopen qstart qend sstart send evalue bitscore qcovhsp scovhsp full_qqual sscinames sphylums.Filenames contain the insect species name and the NCBI accession number of the analyzed SRA library.