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Plasmids and yeast strains.
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创建时间：
2015-12-02
相关数据集
Predicted expression of the of lux genes before and after codon-optimization.
GENSCAN translation prediction scores for expression of wild-type (wt) and codon-optimized (co) lux genes in a mammalian host cell (http://genes.mit.edu). Predicted start position is the predicted nuc
NIAID Data Ecosystem50
Additional file 2 of LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites
Additional file 2: Table S3. The additional excel spreadsheet contains primer sequences, adapter sequences and example sample sheets.
NIAID Data Ecosystem20
Oligonucleotide primers used in the amplification of the protein coding sequences in this study.
The underlined nucleotides indicate overlapping base pairs with the generic primers for the ORFs cloned using the Gateway® system. The bold underlined nucleotides show the restriction site for the app
NIAID Data Ecosystem20
Plasmid list with primer pairs to make the plasmids.
Plasmid list with primer pairs to make the plasmids.
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Efficiency of cloning of pX-GFP at different restriction-ligation times.
Restriction/ligations were performed from 5 to 30 minutes, and either immediately transformed in E.coli (Digest an heat inactivation/no) or incubated 5 minutes at 50°C and 5 minutes at 80°C (Digest an
NIAID Data Ecosystem20
