Taenia crassiceps excreted/secreted products alters the microRNA profile and regulates the pro-inflammatory response of M1 macrophages [miRNA]

We tested the ability of helminth Taenia crassiceps parasite excreted/secreted products (TcES) to induce a regulatory-associated profile in bone-marrow derived macrophages (BMDMs). We also compared gene expression and microRNA profile of BMDMs exposed to TcES with those exposed to LPS which elicits a well-known inflammatory program. Mature macrophages were exposed for 4 or 24 hr to LPS (1 ug/ml), T. crassiceps excreted/secreted products (25ug/ml) or T. crassiceps excreted/secreted products plus LPS. After these time points, total RNA were collected. Next, 15ng of mRNA or microRNA from each group were hybridized overnight following manufacturer's instructions and gene expression was compared to unstimulated BMDMs.