A flowcytometric analysis to efficiently quantify multiple innate immune cells and T cell subsets in clinical studies

To establish a protocol for an advanced multi-colour flow cytometry approach facilitating a reliable and comprehensive description of the cellular immune response of both myeloid and lymphoid cell populations in human blood
Conclusion:
We have established two antibody panels for a comprehensive description of innate and T cell populations. With our method it is possible to evaluate immune activation versus immunosuppression and to receive clear resolution for all 15 markers stained in each panel. The protocol has been optimised for RBC-lysed human whole blood as well as PBMCs.