Raw dataset for the production of antioxidant exopolysaccharides produced by Bacillus amyloliquefaciens KW8 using cassava pulp and nitrogen supplement optimized via Response Surface Methodology

This raw dataset reflects an evaluation of the optimal nitrogen source for the production of antioxidant exopolysaccharides (EPSs) by Bacillus amyloliquefaciens KW8 using cassava pulp carbon source. Various nitrogen sources were initially screened, and yeast extract was identified as the most effective. Further optimization was conducted using Response Surface Methodology (RSM) with a Central Composite Design (CCD) to assess the influence of key parameters, including pH, inoculum size, carbon-to-nitrogen (C/N) ratio, and agitation speed on EPS production. The amount of EPS, media pH, reducing sugar level, and viable bacterial population after 3 days of fermentation were recorded. The EPS's structural characteristics were investigated using scanning electron microscopy (SEM). The levels of bioactive compounds and antioxidant capabilities in the EPS, such as its total phenolic content, total flavonoid content, ferric reducing antioxidant power, hydroxyl radical scavenging activity, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were also measured. This experiment used microbial cell factories to convert cassava pulp, an agro-biowaste, into more valuable EPS bioproducts, which supports sustainable developmental goals.本原始数据集围绕以木薯渣为碳源，利用解淀粉芽孢杆菌（Bacillus amyloliquefaciens）KW8生产抗氧化胞外多糖（exopolysaccharides, EPSs）的最优氮源展开评估。研究团队首先对多种氮源进行筛选，最终确定酵母提取物为效果最佳的氮源。后续采用响应面法（Response Surface Methodology, RSM）结合中心复合设计（Central Composite Design, CCD）开展优化实验，以评估pH值、接种量、碳氮比（C/N）及搅拌转速等关键参数对EPS产量的影响。研究记录了发酵3天后的EPS产量、培养基pH值、还原糖水平以及活菌总数。通过扫描电子显微镜（scanning electron microscopy, SEM）对EPS的结构特征进行了表征分析。同时测定了EPS中的生物活性化合物含量与抗氧化性能，包括总酚含量、总黄酮含量、铁还原抗氧化能力、羟基自由基清除活性以及2,2-二苯基-1-苦肼基（2,2-diphenyl-1-picrylhydrazyl, DPPH）清除活性。本实验以微生物细胞工厂为载体，将农业生物质废弃物木薯渣转化为高附加值的EPS生物制品，契合可持续发展目标。