Highly conserved IESs that are transcribed during MAC development and/or associated to genes that are up-regulated during MAC development.

The transcription level of IESs is indicated for different stages during MAC development (S, T0 to T45) and in vegetative cells (V) [36]. Only P. tetraurelia IESs are shown in the table, because this is the only species for which developmental transcriptome data are available [36]. (*) The IES pte.MICA.16.324097 (FAM_4968) contains a complete protein-coding gene, which is expressed during development (HTH CenpB-type DNA-binding domain; see Fig 5A). The gene in which this IES is inserted (PTET.51.1.P0160202) is not specifically expressed during development. IES, internal eliminated sequence; MAC, macronucleus. (XLSX)