Region-Specific Transcriptional Signatures of Brain Aging in the Absence of Neuropathology at the Single-cell Level

Single nuclear RNA-sequencing was performed on human post-mortem brain tissue from four distinct regions that have implications for neurodegenerative diseases, including Alzheimer's and Parkinson’s diseases. Younger, aged 20s to 30s, and middle-aged and older, aged 60s to 80s, adults were used to identify changes in cell populations and transcriptomes during aging. Files included:aging.glmmtmb_age_diffs.csv.gz - All results modeled using glmmTMBaging.glmmtmb_age_diffs_fdr.csv.gz - glmmTMB results with a statistically significant result Column headers for the glmmtmb results are:feature - gene nameintercept - regression interceptestimate - regression coefficientstd.error - standard errorstatistic - regression test statisticp.value - regression p-valuetissue - the cell-type (varying specificty, use in combinationfor type column)type - type for 'tissue' column; 'broad_celltype', 'region_broad_celltype', or 'specific_celltype'fdr_bh - B&H FDR multiple-test corrected p-value aging.diffxpy_age_diffs.csv.gz - All results using diffxpy differential expressionaging.diffxpy_age_diffs_fdr.csv.gz - diffxpy results with a statistically significant result Column headers for the diffxpy results are:gene - gene namepval - p-value for differential expressionqval - q-value for differential expression; multiple-test corrected p-valuelog2fc - log base 2 fold change in expressionmean -zero_mean -zero_variance -tissue - the cell-type (varying specificty, use in combinationfor type column)type - type for 'tissue' column; 'broad_celltype', 'region_broad_celltype', or 'specific_celltype'fdr_bh - B&H FDR cmultiple-test orrected p-value aging.h5ad - AnnData containing the demultiplexed unprocessed/non-transformed quantified expression data. aging.pegasus.leiden_085.subclustered.h5ad - AnnData containing the fully processed, transformed, clustered, and cell-type labeled expression data.