Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12 [I]

High-endothelial-venules (HEVs) offer another entry point for tumor metastasis in mice. To investigate whether HEVs and non-specialized blood vessels exert different effects on disseminating tumor cells, we enriched EO771 derivatives HEVM3 (preferentially metastasize through HEVs) cells and BVM3 (preferentially metastasize through blood vessels) cells. To further explore the lymph node metastasis of breast tumor cells, we selected the primary tumors and paired lymph nodes from six C57BL/6J mice were analyzed with single-cell RNA sequencing. Overall design: The primary tumors (EO771-HEVM3) and paired lymph nodes from six C57BL/6J mice were analysed with single-cell RNA sequencing.