Dendrobaena veneta earthworm - antiplatelet effect

The present work aimed to assess whether the different concentrations of the coelomic fluid of D. veneta may affect human platelets and blood coagulation. List of used abbreviations:PBS - Phosphate Buffered SalineASA – aspirinEkstrakt stęż. 25 – extract with a concentration of 25 µg/mLEkstrakt stęż. 50– extract with a concentration of 50 µg/mLEkstrakt stęż. 100 – extract with a concentration of 100 µg/mLASPI – ASPItest, aggregation in response to 0.5 mM arachidonic acid (aggregation in response to 0.5 mM arachidonic acid)ADP – ADPtest, platelet aggregation in response to 6.5 µM adenosine-5′-diphosphateTRAP – TRAPtest, aggregation in response to 32 µM thrombin receptor activating peptide 6 (aggregation in response to 32 µM thrombin receptor activating peptide 6)PT – prothrombin timeU, UA – unit used in ASPItest, ADPtest, TRAPtestINR – International Normalized Ratio, used to monitor the effectiveness of anticoagulantsPR - protrombin ratio (%)Equipment used for ASPI, ADP, TRAP tests: Multiplate® Platelet Function Testing 5.0 Analyzer (Roche, Rotkreuz, Switzerland