Raw data for the article “TsrF, a transcriptional regulator in clinical Pseudomonas aeruginosa, modulates H4-T6SS expression and bacterial virulence”

This dataset contains the raw experimental data related to TsrF-mediated regulation of H4-T6SS and virulence in the clinical Pseudomonas aeruginosa strain LYSZa7, including:Transcriptional level data of T6SS-related genes (tssB4, tssH4, amrZ, fleQ, etc.) detected by RT-qPCR;Original bands and gray-scale quantitative data of TssB4 protein expression detected by Western blot;Raw results and statistical analysis data from antibacterial competition assays, biofilm formation assays, A549 cell cytotoxicity assays, and Galleria mellonella infection assays;Raw RNA-seq data (deposited in NCBI BioProject under accession number PRJNA1372908).All data are the supporting raw data for the paper “TsrF, a transcriptional regulator in clinical Pseudomonas aeruginosa, modulates H4-T6SS expression and bacterial virulence” and can be used for result verification and subsequent research reuse.