Human bladder,colon,kidney,liver cancer LC MS/MS

We propose that enrichment of low-abundance proteins benefits MPs finding. ProteoMinerTM is an equalizing technique by reducing high-abundance proteins and enriching low-abundance proteins in biological liquids. With triton X-100/TBS buffer extraction, ProteoMinerTM enrichment and peptide fractionation, 25 MPs with 73 unique peptides were identified from four human tissues, including 10 membrane proteins and 6 nucleus proteins. Then 18 MPs were confirmed with at least 2 unique peptide identification and 6 MPs were confirmed with 1 unique peptide identification by PRM assay. Hence these results demonstrated ProteoMinerTM as a powerful means in discovery of MPs.