Oxygen-deficit history predicts biological recovery debt after dissolved-oxygen compliance in managed freshwaters

This dataset and code repository supports the manuscript “Oxygen-deficit history predicts biological recovery debt after dissolved-oxygen compliance in managed freshwaters”. The study combines high-frequency dissolved-oxygen monitoring, a controlled prior oxygen-history recovery experiment in juvenile bighead carp and grass carp, and independent managed-waterbody low-oxygen–reoxygenation event validation. The repository contains processed data, source tables, model outputs, codebook files and R scripts used to reproduce the oxygen-history descriptors, recovery-debt indices and external validation model comparison reported in the manuscript. The deposited files include field dissolved-oxygen logger summaries, oxygen-deficit duration and cumulative deficit-load metrics, achieved exposure and water-quality tables, respirometry-derived recovery-debt data, systemic physiological records, metabolic and branchial recovery-debt summaries, targeted protein-level stress-response summaries, integrated recovery-debt index outputs, independent external validation stage-level input data and Table 3 model-comparison outputs. The primary external validation model comparison uses 20 event/reference-window × sampling-stage summaries. Fish-level Field RDI records were used to estimate stage-level means and uncertainty, but were not used as independent rows in the primary AICc, CV-RMSE, leave-one-event-out CV-RMSE, marginal R² or calibration-slope model comparison.本数据集与代码仓库配套支撑论文《溶解氧达标后人工淡水生态系统中缺氧历史可预测生物恢复负债》（原文标题："Oxygen-deficit history predicts biological recovery debt after dissolved-oxygen compliance in managed freshwaters"）。本研究整合了高频溶解氧监测（high-frequency dissolved-oxygen monitoring）、针对幼体鳙鱼与草鱼的受控缺氧历史恢复对照实验，以及独立人工水体低氧-复氧事件验证。 本仓库包含用于复现论文中报道的缺氧历史表征指标、恢复负债指数（recovery-debt indices）及外部验证模型对比的预处理数据、源数据表、模型输出文件、代码手册文件与R脚本。所提交的文件包括野外溶解氧记录仪汇总数据、缺氧持续时长（oxygen-deficit duration）与累积亏氧量（cumulative deficit-load）指标、实测暴露量与水质数据表、呼吸测定法（respirometry）衍生的恢复负债数据、全身生理记录、代谢与鳃组织（branchial）恢复负债汇总、靶向蛋白水平应激响应汇总、整合恢复负债指数输出、独立外部验证阶段级输入数据，以及表3的模型对比输出结果。 本次外部验证的核心模型对比采用了20组事件/参考窗口×采样阶段汇总数据。本研究使用鱼类水平的野外恢复负债指数（RDI）记录来估算阶段级均值与不确定性，但未将其作为独立样本行纳入核心的修正赤池信息准则（AICc）、交叉验证均方根误差（CV-RMSE, Cross-Validation Root Mean Square Error）、留一事件交叉验证均方根误差（leave-one-event-out CV-RMSE）、边际决定系数（marginal R²）或校准斜率（calibration-slope）模型对比分析。