Microsatellite genotypes for adult and seedlings of the temperate seagrass (ribbon weed), Posidonia australis, from four meadows at Rottnest Island, Western Australia

Adult shoots and seedings of the ribbon weed (Posidonia australis Hook.f.), a widespread temperate seagrass, were sampled from four meadows around Rottnest Island, Western Australia. The data set contains multilocus genotypes for adult shoots from four meadows and seedlings from three meadows over two consecutive years. The metadata file contains: Pop number (1 – 10), Sample site, Latitude (S), Longitude (E), individual sample code, year of sampling, life stage (adult shoot or seedling), genotypes (2 columns per locus). The seven polymorphic microsatellite loci are: PaA1, PaA105, PaA120, PaB6, PaB8, PaB112, PaD113. Most genotypes are diploid, however, 3N genotypes are included., Fifty adult P. australis shoots were collected from randomly generated GPS coordinates within a 50 m diameter circle from four meadows around Rottnest Island: Thomson Bay (RTB), Stark Bay (RST) and Nancy Cove (RNC) and Parker Point (RPP). Approximately 30 one year old P. australis seedlings were also collected for genotyping following annual surveying for two consecutive years (2014 an 2015). All adult shoot and seedling samples were genotyped for seven polymorphic DNA loci (PaA1, PaA105, PaA120, PaB6, PaB8, PaB112, PaD113). Laboratory and genotyping methods are described in full in Sinclair et al. (2014b).
Clonal and genetic diversity indices were estimated for all adult shoots and seedling cohorts using Genalex version 6.5 (Peakall & Smouse, 2012). The ‘Find Clones’ option was used to identify shared multilocus genotypes (MLGs) within and among meadows. PID was then used to determine whether shared MLGs were considered to come from the same vegetative clone or a separate recruitme..., ,