Microsatellite genotypes for adult and seedlings of the temperate seagrass (ribbon weed), Posidonia australis, from four meadows at Rottnest Island, Western Australia

Adult shoots and seedings of the ribbon weed (Posidonia australis Hook.f.), a widespread temperate seagrass, were sampled from four meadows around Rottnest Island, Western Australia. The data set contains multilocus genotypes for adult shoots from four meadows and seedlings from three meadows over two consecutive years. The metadata file contains: Pop number (1 – 10), Sample site, Latitude (S), Longitude (E), individual sample code, year of sampling, life stage (adult shoot or seedling), genotypes (2 columns per locus). The seven polymorphic microsatellite loci are: PaA1, PaA105, PaA120, PaB6, PaB8, PaB112, PaD113. Most genotypes are diploid, however, 3N genotypes are included., Fifty adult P. australis shoots were collected from randomly generated GPS coordinates within a 50 m diameter circle from four meadows around Rottnest Island: Thomson Bay (RTB), Stark Bay (RST) and Nancy Cove (RNC) and Parker Point (RPP). Approximately 30 one year old P. australis seedlings were also collected for genotyping following annual surveying for two consecutive years (2014 an 2015). All adult shoot and seedling samples were genotyped for seven polymorphic DNA loci (PaA1, PaA105, PaA120, PaB6, PaB8, PaB112, PaD113). Laboratory and genotyping methods are described in full in Sinclair et al. (2014b). Clonal and genetic diversity indices were estimated for all adult shoots and seedling cohorts using Genalex version 6.5 (Peakall & Smouse, 2012). The ‘Find Clones’ option was used to identify shared multilocus genotypes (MLGs) within and among meadows. PID was then used to determine whether shared MLGs were considered to come from the same vegetative clone or a separate recruitme..., ,本研究从澳大利亚西部罗特内斯特岛周边4片海草床中，采集了澳洲波喜荡草（Posidonia australis Hook.f.，俗称带形海草）的成株与幼苗——该物种为广布性温带海草。本数据集涵盖连续两年间，4片海草床成株与3片海草床幼苗的多位点基因型数据。 元数据文件包含以下信息：种群编号（1~10）、采样位点、纬度（南纬）、经度（东经）、个体采样编码、采样年份、生活型（成株或幼苗）、基因型（每个位点对应2列数据）。 所用的7个多态性微卫星位点为：PaA1、PaA105、PaA120、PaB6、PaB8、PaB112、PaD113。多数基因型为二倍体，同时包含三倍体（3N）基因型。 研究人员从罗特内斯特岛周边4片海草床——汤姆森湾（RTB）、斯塔克湾（RST）、南妮湾（RNC）与帕克角（RPP）——内直径50米的圆形区域内随机生成的GPS坐标点中，采集了50株澳洲波喜荡草成株。在连续两年（2014年与2015年）的年度调查后，还采集了约30株1年龄的澳洲波喜荡草幼苗用于基因分型。 所有成株与幼苗样本均针对上述7个多态性DNA位点完成了基因分型。实验方法与基因分型流程详见Sinclair等人（2014b）的完整报道。 研究采用GenAlEx 6.5版本（Peakall & Smouse, 2012），对所有成株与幼苗群体计算了克隆多样性与遗传多样性指数。 通过‘Find Clones’功能，鉴定了海草床内部及不同海草床间共享的多位点基因型（MLGs）。随后采用个体识别概率（PID）判断这些共享的多位点基因型是否源自同一营养繁殖克隆，或是独立的定植个体……