Raw diffraction images of Drosophila Piwi

Crystal structure of Drosophila Piwi (PDB code: 6KR6). 28 mercury-bound data and 4 native data were included (see file_list.txt for details). Each dataset consists of 180° (except two 90° datasets) from single crystal and was collected using EIGER X 9M detector at a wavelength of 1 Å with helical data collection scheme using 15×10 μm beam on BL32XU, SPring-8. All diffraction images were processed using DIALS 1.10.2 through KAMO pipeline, and merged using XSCALE from XDS package with kamo.multi_merge. The crystals belong to space group P212121 with a=62.1, b=115.6, c=119.9 Å. Finally 23 mercury-bound datasets were merged at 2.9 Å resolution in the published result (Yamaguchi et al. Nature Communications, 2020). Merging improved resolution and electron density of PAZ domain that was difficult to interpret with a single dataset.