SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in March 2011

Flow cytometry data was collected in March 2011, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (FM) layer were investigated. Flow cytometry data is normally collected for picophytoplankton, bacteria and viruses, but not on this cruiseSix main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

流式细胞术(Flow Cytometry)数据采集于2011年3月，采集区域为澳大利亚南部近海海域。本研究的总体目标为：构建澳大利亚南部大陆架海域生态系统的背景知识库，阐明上升流与高盐流出事件对微生物群落的影响，最终建立该区域的生物地球化学模型。采样工作依托恩格林号（RV Ngerin）科考船开展，属于澳大利亚南部综合海洋系统(Southern Australian Integrated Marine System, SAIMOS)项目的组成部分。每航次期间，研究人员均对叶绿素荧光最大值层(chlorophyll fluorescence maximum, FM)的物理、化学与生物学特性开展调查。常规流式细胞术检测通常会针对微微型浮游植物、细菌与病毒采集数据，但本次航次未开展此类检测。本研究共设置6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）与SAM5CB/B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），地处袋鼠岛西南海域。需注意：结合站位间距（14~25海里）、陆架中层海流平均流速分量（0.01 m·s⁻¹）与科考船平均航速（约9节）可知，各站位所采集的水体分属不同水团。研究期间还偶尔采集袋鼠岛国家参考站(National Reference Station, NRS，35.832°S，136.447°E)以及发生高盐流出事件的南澳斯宾塞湾口锚系(SAM8SG，35.25°S，136.690°E)的补充样本。