transcriptional landscape of Germ cell differentiation at single cell resolution

Single cell sequencing technologies are powerful tools for the dissection of large regulatory networks and their role in directing developmental trajectories. The goal of this project is to profile the transcriptional landscape of germ cell development in the mouse male embryo at single cell resolution, and to examine the dynamic regulation of the molecular networks directing epigenetic reprogramming in these cells. Overall design: Single cell RNAseq profiling (10x Genomics) of Primordial Germ Cells isolated via Fluorescence Actvated Cell Sorting, using a germ cell specific Oct4-EGFP fluorescent reporter. One replicate per time point (E10.5 and E13.5)