Multi-behavioral endpoint testing of an 87-chemical compound library in freshwater planarians

There is an increased recognition in the field of toxicology of the value of medium-to-high-throughput screening methods using in vitro and alternative animal models. We have previously introduced the asexual freshwater planarian Dugesia japonica as a new alternative animal model and proposed that it is particularly well-suited for the study of developmental neurotoxicology. In this paper, we discuss how we have expanded and automated our screening methodology to allow for fast screening of multiple behavioral endpoints, developmental toxicity, and mortality. Using an 87-compound library provided by the National Toxicology Program (NTP), consisting of known and suspected neurotoxicants, including drugs, flame retardants, industrial chemicals, polycyclic aromatic hydrocarbons (PAHs), pesticides and presumptive negative controls, we further evaluate the benefits and limitations of the system for medium-throughput screening, focusing on the technical aspects of the system. We show that, i...毒理学领域对体外（in vitro）及替代动物模型的中高通量筛选方法的价值认可度与日俱增。此前我们已将无性系淡水涡虫日本三角涡虫（Dugesia japonica）作为新型替代动物模型引入本研究领域，并提出其尤其适配发育神经毒理学研究。本文探讨了我们如何对筛选方法进行扩展与自动化改造，以实现对多种行为终点、发育毒性及死亡率的快速筛选。本研究使用美国国家毒理学计划（National Toxicology Program, NTP）提供的87种化合物文库，该文库涵盖已知及疑似神经毒物，包括药物、阻燃剂、工业化学品、多环芳烃（PAHs）、农药以及推定阴性对照。借此我们进一步评估了该中高通量筛选系统的优势与局限，重点关注系统的技术层面。本研究证实，……