Effects of endocytic pathway inhibitors on cell entry of rCARDS toxin.
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HeLa cells were pre-treated with 5 mM of Methyl-β-cyclodextrin, 100 µM of Monodansylcadaverine, or 1 µg Filipin for 30 min at 37°C. Subsequently, rCARDS toxin or transferrin or cholera toxin was added individually. Cell entry of these molecules was analyzed by confocal laser scanning immunofluorescence microscopy.
创建时间:
2015-12-02



