The megakaryocytic transcription factor ARID3A suppresses leukemia pathogenesis [CUT&RUN]. The megakaryocytic transcription factor ARID3A suppresses leukemia pathogenesis [CUT&RUN]

The purpose of this study was to decipher the molecular function of ARID3A. We leveraged gene expression (RNA-Seq) and chromatin profiling (ATAC-Seq and CUT&RUN) to evaluate gene expression changes upon restoring Arid3a expression in the context of the Gata1s mutation and miR-125b overexpression Overall design: We induced Arid3a-FLAG protein using doxycycline in Gata1s-FLCs lentivirally expressing miR-125b