Gut Microbiome IMS-MS Experiments

Bile acids play key roles in nutrient uptake, inflammation, signaling, and microbiome composition. While previous bile acid analyses have primarily focused on profiling 5 canonical primary and secondary bile acids and their glycine and taurine bile acid-amino acid (BA-AA) conjugates, recent studies suggest that many other microbial conjugated BA-AAs (or MCBAs) exist. MCBAs are produced by the gut microbiota and serve as biomarkers providing information about early disease onset and gut health. Here we analyzed 8 core bile acids synthetically conjugated with 22 proteogenic and non-proteogenic amino acids totaling 176 MCBAs to make an MCBA library, and applied this library to evaluate two model systems.