Full-length RNA transcript sequencing traces brain isoform diversity in house mouse natural populations

Using a newly developed full-length transcripts enrichment protocol with 5’ CAP selection, we sequenced full-length RNA transcripts of 48 individuals from outbred populations and subspecies of Mus musculus, and from the closely related sister species Mus spretus and Mus spicilegus as outgroups. The dataset represents the most extensive full-length high-quality isoform catalog at the population level to date.

采用一项新近研发的全长转录本富集协议，结合5' CAP选择技术，我们对48名来自Mus musculus的杂交种群及其亚种，以及与之密切相关的姐妹物种Mus spretus和Mus spicilegus的全长RNA转录本进行了测序。该数据集构成了迄今为止在种群层面上最全面、高质量的同源变异表型目录。