Figure 6_raw data

Raw Image data that were analysed to be presented in Figure 6. If image data is not of interest, see https://doi.org/10.5683/SP2/0SHJNT where we uploaded the final data plotted in Figure 6. Here see \"Figure6_SummarizeColocImageData.xlsx\" for summary of Image data including cell line used, BH3 mimetics tested and size of datasets. This helps navigate the subfolders to find the images of interest. Data was organised by date of the experiment. Within each folder see, '.Tiffs' exported from PerkinElmer Harmony software. Files names for example \"r02c02f01p01-ch1...\" indicating the image is from row 2, column 2, field of view 1, position 1 and channel 1. Within each folder see \"Analysis\" Sub folder where one can find: -\"SaveImagingConfiguration.xlsx\" where we saved the imaging settings from the Phenix microscope. The objective lens, excitation/emission settings for channels 1,2,3,4 (in order that they appear), and number of fields of view acquired for that experiment can be found here. -.xlsx file with \"platemap\" included in filename. Shows detailed map of Cells/Treatment within 384 well plate. Use Row/Column information to navigate the image data. -Subfolder labelled \"CP_results\" : include results the object-level (single cell) results exported from Cell Profiler. These data were plotted separately in GraphPad Prism (see .pzf file) to determine the states o from each replicate. Then, median values were plotted in Figure 6 from 3+ replicates. See \"Figure6_CombinedFinalColocResults.xlsx\" where we combine the final results ultimately plotted in Figure 6.本数据集为用于生成图6的原始图像分析数据。若无需关注原始图像内容，可访问链接https://doi.org/10.5683/SP2/0SHJNT，此处已上传用于绘制图6的最终数据。可通过"Figure6_SummarizeColocImageData.xlsx"查看图像数据汇总信息，涵盖所用细胞系、检测的BH3模拟物（BH3 mimetics）以及数据集规模，可帮助您快速定位目标子文件夹以获取所需图像。本数据集按实验日期进行组织。每个实验文件夹内均包含由PerkinElmer Harmony软件导出的.TIFF格式图像文件。文件名示例为"r02c02f01p01-ch1..."，其命名规则表明该图像取自第2行、第2列、视野1、点位1及通道1。每个实验文件夹内设有"Analysis"子文件夹，其中包含以下内容：1. "SaveImagingConfiguration.xlsx"：记录了Phenix显微镜的成像参数，包括物镜参数、通道1至4（按显示顺序）的激发/发射设置，以及本次实验采集的总视野数。2. 文件名包含"platemap"（孔板布局图）的.xlsx文件：详细标注了384孔板内的细胞接种与处理布局，可通过行列信息快速检索对应图像数据。3. 名为"CP_results"的子文件夹：包含由Cell Profiler导出的单物体（单细胞）级分析结果。上述数据已通过GraphPad Prism单独绘图（详见.pzf文件）以确定各重复样本的细胞状态，最终取3次及以上重复的中位数值用于绘制图6。可通过"Figure6_CombinedFinalColocResults.xlsx"查看用于最终绘制图6的合并分析结果。