Effects of deletioin of cholesterol efflux genes Abca1 and Abcg1 on other gene expressions in macrophages

ATP binding cassette subfamily member 1 (ABCA1) and G1 (ABCG1) are cholesterol efflux transporter to prevent excess intracellular cholesterol accumulation. We here report the deletion of Abca1 and Abcg1 results in the significant increased expression of Cd38, a multi-faceted ectoenzyme with NADase activity. Overall design: To investigate the effect of the deletion of Abca1 and Abcg1 on macrophages, bone marrow (BM) cells isoleted from the sex-matched littermate of Abca1/Abcg1 flox/flox and Abca1/Abcg1 flox/flox LysM-Cre mice were collected. Bone marrow derived macrophages (BMDM) were obtained by differentiating from BM cells.