Multilevel Stressors and Systemic and Tumor Immunity in Black and White Women With Breast Cancer

A cross-sectional study was conducted between 2012 and 2023 to collect breast tumor and tumor-adjacent normal tissue (N=48 tumor, 41 tumor-adjacent normal) from women with breast cancer. Participants were recruited through the University of Maryland Medical Center and other hospital sites within the area of Baltimore, Maryland. RNA-sequencing and whole exome sequencing was performed on these fresh-frozen tissues. Genomic DNA was extracted from frozen breast tumors and adjacent normal tissue using the Qiagen DNeasy Blood & Tissue kit and checked for quality using the Agilent Genomic DNA Screen tape assay. WES was performed by the service provider Psomagen and data processed as in Tang, et al, 2023. Overall design: RNA was isolated from frozen breast tumors using TRIzol method. The mRNA profiles of the above breast tumors were generated by deep sequencing using Illumina NovaSeq 6000.