mNGS of hemophagocytic lymphohistiocytosis patients' plasma Raw sequence reads

In order to find out what pathogen infection in hemophagocytic lymphohistiocytosis patients, blood samples collected from each patient to separate the plasma. Extracted DNA was sonicated and 200-300 bp DNA fragments were used to construct DNA libraries through DNA fragmentation, end repair, adapter ligation, and PCR amplification. Qualified libraries were pooled and DNA nanoballs (DNBs) made using single-stranded DNA circles were sequenced using a MGISEQ-2000 platform. High-quality sequencing data were generated by removing low-quality reads and human host sequences mapped to the human reference genome (ftp://ftp.ncbi.nlm.nih.gov/genome) were removed. The remaining sequence data were aligned with the genomes of microbes associated with human diseases, including 2,328 bacteria, 199 fungi, 4,189 viruses, 135 parasites, 83 mycobacteria, and 41 mycoplasma/Chlamydia.