Normalized transcriptomic read counts.

The expression of each gene after normalization (see Materials and methods) is tabulated as read counts. Untreated cultures of wild-type L. biflexa, are compared to cultures treated with 5-aminolevulinic acid (ALA) or with the iron-chelator 2,2’-dipyridyl (DIP). Independent replicas are labeled with indices 1, 2 and 3 on the columns’ names. Note there are 9 different sheets in the Excel table: the first two (Sheets 1 and 2) correspond to normalized read counts for the wt and the ΔhemKR strains respectively, with column names marking whether they come from untreated samples or following treatment with ALA or DIP. Sheets 3 and 4: Differential Gene Expression (DGE) analyses comparing ALA vs untreated conditions, respectively for the wt and ΔhemKR mutant strains. Sheets 5 and 6: DGE analyses comparing DIP vs untreated conditions, respectively for the wt and ΔhemKR mutant strains. Sheets 7–9: DGE analyses comparing expression of ΔhemKR mutant vs wt strains, respectively with no treatment, treated with DIP, and treated with ALA. (XLSX)