MOESM4 of Metagenomic analysis and functional characterization of the biogas microbiome using high throughput shotgun sequencing and a novel binning strategy

Additional file 4. Input files used for the network Representation of the Biogas Functional Organization (NRBFO). Functional analysis of the microbial community begins with the identification of the GBs enriched in SEED subsystem counts (ranked among the top one eighth of each SEED category). Starting from the entire list of SEED sub-categories (Data Set 6) 56 were selected (the more relevant for the characterization of the biogas community) and the GBs with high number of subsystem counts were identified (“node abundance” worksheet). The subsystem categories were compared in order to identify if they shared the same GBs and the couples with three or more common genome bins were identified (“edge_size_connections” worksheet). The number of GBs identified for each category was used to set the node size and the number of GBs shared was used to set the edge size (Fig. 2).