Identification of visual cortex transcriptome of Mmp9 UTRmut mice (B6.CBA Mmp9em1Iimcb/Tar)

As microRNAs have emerged as important regulators of molecular events occurring at the synapses, new questions about their regulatory effect on the behavior have arisen. In the present study, we elucidated the physiological consequences of miR132-Mmp9 mRNA interaction in vivo. To address this question, we created a new mouse model with a disrupted miR132 binding site in Mmp9 mRNA 3’UTR. In the current manuscript, we show for the first time that the dysregulated specific targeting of miR132 to Mmp9 mRNA in the mouse brain results in the increased level of Mmp9, which affects synaptic plasticity and has an effect on mice behavior. Our data points at the importance of complex and precise regulation of Mmp9 level by miR132 in the brain. Examination of mouse visual cortex transcriptome in Mmp9UTR mut, heterozygous and WT mice, performed in two replicates.