Nanoarchitectonics of doxycycline-loaded vitamin E–D-α-tocopheryl polyethylene glycol 1000 succinate micelles for ovarian cancer stem cell treatment - supplementary data

<b>Aim: </b>This study aim to develop doxycycline within the D-<b>α</b>-tocopheryl polyethylene glycol 1000 succinate micelle platform as an anticancer stem cell agent. <b>Materials &amp; methods: </b>The optimized nanomicelle formulation was prepared using the solvent casting method and evaluated through physicochemical and biological characterization. <b>Results: </b>Nanomicelles exhibited mean particle sizes of 14.48 nm (polydispersity index: 0.22) using dynamic light scattering and 18.22 nm using transmission electron micrography. Drug loading and encapsulation efficiency were 2% and 66.73%, respectively. Doxycycline-loaded micelles exhibited sustained release, with 98.5% released in 24 h. IC50 values were 20 μg<b>/</b>ml for free drug and 5 μg<b>/</b>ml for micelles after 48 h of cell exposure. A significant 74% reduction in CD44 biomarker and 100% colony formation inhibition were observed. <b>Conclusion: </b>Doxycycline in hemo<b>/</b>biocompatible nanomicelles holds potential for ovarian cancer stem cell therapy.