Transcriptomes of human trained macrophages before and after Mtb exposure

Trained Immunity (TRIM) is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to specific microbial or metabolic components. In this study, monocytes were trained with beta-glucan and then re-exposed to Mycobacterium tuberculosis. In this study human monocytes were exposed to beta-glucan for 24 hours in vitro, a stimulus that induced TRIM, and were then differentiated to macrophages for another 5 days. At day 6, trained and not-trained macrophages were exposed to Mycobacterium tuberculosis (Mtb) for 4 hours and their transcriptomes were profiled .