mRNA expression profile in the liver after overexpressed tsRNA-Glu-CTC oligos in C57BL/6 mice with mRNA-seq method

Purpose: To reveal mRNA profiles in the liver using mRNA-seq method. Methods: 8 week old C57BL/6 male mice were injected Control Oligos and tsRNA-Glu-CTC oligos for two weeks. Liver RNA was extracted with Trizol methods. Results:mRNA-seq revealed upregulation of lipids metabolism related genes in overexpressed tsRNA-Glu-CTC oligos mice. Importantly, cholesterol synthesis key genes SREBP2 and HMGCR are significantly upregulation. Overall design: Liver mRNA were sequenced from C57BL/6 male mice with I.P. injection of Control Oligo and tsRNA-Glu-CTC Oligo-1 for two weeks