Sohn2010 - Genome-scale metabolic network of Pichia pastoris (PpaMBEL1254)

Sohn2010 - Genome-scale metabolic network of
Pichia pastoris (PpaMBEL1254)

This model is described in the article:

Genome-scale metabolic model
of methylotrophic yeast Pichia pastoris and its use for in
silico analysis of heterologous protein production.

Sohn SB, Graf AB, Kim TY, Gasser B,
Maurer M, Ferrer P, Mattanovich D, Lee SY.
Biotechnol J 2010 Jul; 5(7):
705-715
Abstract:

The methylotrophic yeast Pichia pastoris has gained much
attention during the last decade as a platform for producing
heterologous recombinant proteins of pharmaceutical importance,
due to its ability to reproduce post-translational modification
similar to higher eukaryotes. With the recent release of the
full genome sequence for P. pastoris, in-depth study of its
functions has become feasible. Here we present the first
reconstruction of the genome-scale metabolic model of the
eukaryote P. pastoris type strain DSMZ 70382, PpaMBEL1254,
consisting of 1254 metabolic reactions and 1147 metabolites
compartmentalized into eight different regions to represent
organelles. Additionally, equations describing the production
of two heterologous proteins, human serum albumin and human
superoxide dismutase, were incorporated. The protein-producing
model versions of PpaMBEL1254 were then analyzed to examine the
impact on oxygen limitation on protein production.

This model is hosted on
BioModels Database
and identified by:
MODEL1507180050.
To cite BioModels Database, please use:
BioModels Database:
An enhanced, curated and annotated resource for published
quantitative kinetic models.

To the extent possible under law, all copyright and related or
neighbouring rights to this encoded model have been dedicated to
the public domain worldwide. Please refer to
CC0
Public Domain Dedication for more information.