16S rRNA sequencing data of intestinal flora

The aim of this study was to determine the effects of repeated sevoflurane (Sev) exposure and the possible role of gut-brain axis. Thirty 28-day Sprague-Dawley rats were randomly divided into five experimental groups.The Sev group was exposed to 3.2 % sevoflurane for 2 h on postnatal day (P) 35, P42, and P49. The Sev-Con group only received carrier gas for 2 h at the same time points. The ANT group received antibiotic pre-treatment only since P28 for 3 weeks. The Sev+ANT group was treated with antibiotics for 3 weeks and then exposed to 3.2% sevoflurane for 2 h at each time point. Control group (Con group): animals were freely raised without special treatment.Finally, the 16S rRNA sequencing indicated that at the genus level, Ligilactobacillus, Faecousia, and Alloprevotella showed an increasing trend, while Lactobacillus showed a decreasing trend. The results may reflect that multiple exposures to Sev anesthesia may induce or aggravate cognitive impairment through the gut-brain axis at the developmental stage.