Dazl HITS-CLIP on 25dpp C57BL/6 mouse testes

The autosomal gene Dazl is a member of highly conservative DAZ family, all of which contains a RNA binding domain and expresses in germ cells. In mice, Dazl plays an essential role in germ cell development, germ cell lost in Dazl knockout mouse on pure C57BL/6 strain background. Here we use HITS-CLIP to globally characterize the genome-wide target RNA of Dazl in mouse testes. Sequencing data provides large quantities of clues to help us to get close to the truth of Dazl function. We find over 1700 RNAs are bind by Dazl mainly at 3'UTR and the genes associated with transcription, RNA splicing and reproductive cellular process are enriched significantly. Overall design: We performed 2 biological duplicate, every duplicate used 4 testes from 4 wild type C57BL/6 25dpp mouse. We successfully identified Dazl-RNA complex at 33kDa and radiolabel RNA was detective at 33kDa and about 40kDa smear shift. We constructed two independent cDNA libraries from two 33kDa samples, which is sample 1 and sample 2, and mixed two 40kDa samples together to be one, sample 3. At the same time, we also performed RNA-seq of total 25dpp mouse testes as input background.