Differential population of the mRNA transcripts induced by 24-epibrassinosteroid (EBR) in phloem sap of cucumber plants.

EBR-induced changes in the population of mRNAs in the phloem sap of cucumber plants were determined by suppressive subtractive hybridization (SSH) methods. Mature leaves of seven-leaf stage plants were treated with distilled water (DW) or 0.2 µM EBR and phloem sap was collected from stem cut close to apex and at petioles during various time intervals (3, 6, 9 and 24 h) after treatment. RNA was extracted from pooled samples for use in SSH analysis. Data presented reflects the mRNA species up-regulated in EBR-treated vs DW-treated phloem sap.