Malaria-specific Type 1 Regulatory T cells are more abundant in first pregnancies and associated with placental malaria [PBMCs RNA-seq]

To investigate Tr1 cells and TNFa single expressing cells we stimulated PBMCs from primigravid women with VAR2CSA expressing P. falciparum and sorted CD4+ T cells expressing both IL10 and IFNg or only TNF Overall design: Cytokine capture assay CD4+ T cells expressing either both IL10 and IFNg or only TNFa.