Data from: Metabolomic perfusate analysis during kidney machine perfusion: the pig provides an appropriate model for human studies

Introduction: Hypothermic machine perfusion offers great promise in kidney
transplantation and experimental studies are needed to establish the
optimal conditions for this to occur. Pig kidneys are considered to be a
good model for this purpose and share many properties with human organs.
However it is not established whether the metabolism of pig kidneys in
such hypothermic hypoxic conditions is comparable to human organs.
Methods: Standard criteria human (n = 12) and porcine (n = 10) kidneys
underwent HMP using the LifePort Kidney Transporter 1.0 (Organ Recovery
Systems) using KPS-1 solution. Perfusate was sampled at 45 minutes and 4
hours of perfusion and metabolomic analysis performed using 1-D 1H-NMR
spectroscopy. Results: There was no inter-species difference in the number
of metabolites identified. Of the 30 metabolites analysed, 16 (53.3%) were
present in comparable concentrations in the pig and human kidney
perfusates. The rate of change of concentration for 3-Hydroxybutyrate was
greater for human kidneys (p<0.001). For the other 29 metabolites
(96.7%), there was no difference in the rate of change of concentration
between pig and human samples. Conclusions: Whilst there are some
differences between pig and human kidneys during HMP they appear to be
metabolically similar and the pig seems to be a valid model for human
studies.