Harnessing extracellular vesicles from adipose-derived stem cells for the treatment of 4-vinylcyclohexene diepoxide-induced premature ovarian insufficiency

Adipose-derived stem cells (ADSCs) demonstrated potential in tissue repair, and their extracellular vesicles (ADSC-EVs) show more safety in clinical translation. This study aims to investigate the protective effects of ADSC-EVs on rat POI models and to explore the potential therapeutic mechanisms. Whole-transcriptome sequencing of ADSC-EVs were performed to identify bioactive molecules. In this study, EVs were extracted and purified from the culture medium of adipose-derived mesenchymal stem cells isolated from rats. A total of three batches of extracellular vesicles were obtained from the cell samples for the exploration of potential active RNA components in ADSC-EVs.