首页/数据集/Engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus [miRNA]

Engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus [miRNA]

ChEBI2015-05-07 更新2026-07-21 收录

基因编辑

RNA干扰抗病毒机制

数据链接：

https://www.ebi.ac.uk/biostudies/ArrayExpress/studies/E-GEOD-68620 数据链接链接失效反馈

官方服务：

资源简介：

Engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus [miRNA]

应用场景：

创建时间：

2015-05-07

基因编辑

生物医学研究

CRISPR-stop技术将蛋白质编码序列转化为终止密码子，从而在适当的位置产生了无效等位基因。CRISPR-stop在单细胞期合子中诱导产生Founder 0（F0）纯合突变体小鼠，这避免了小鼠繁殖并可作为非致命基因的快速筛选方法。然而哺乳动物25%的基因，其功能丧失会导致早期死亡。在这里，我们在二细胞期合子中的一个细胞进行CRISPR-stop操作，一种我们称之为嵌合型CRISPR-stop的

中国科学院脑科学数据中心2023-12-11 更新140

CRISPR Trans-Epigenetic Gene Activation _ MiSeq data

基因编辑

高通量测序

MiSeq analysis of the ratio of indel generated by CRISPR/Cas9

Mendeley Data2024-03-27 更新70

Methods for plant transformation

植物转基因

基因编辑

The methods disclosed herein use a morphogenic gene or a repressor gene to provide a transgenic plant containing a single copy of a transgenic event wherein the morphogenic gene or the repressor gene

国家林业和草原科学数据中心2023-02-12 更新70

ENGINEERED NUCLEASES TO GENERATE MUTATIONS IN PLANTS

基因编辑

植物改良

Methods are provided to mutate， in a targeted manner， the genome of a plant cell using a double stranded DNA break inducing enzyme. Also provided are plants， in particular Brassica plants that yield

国家林业和草原科学数据中心2023-02-12 更新80

Additional file 3 of Utilizing AAV-mediated LEAPER 2.0 for programmable RNA editing in non-human primates and nonsense mutation correction in humanized Hurler syndrome mice

基因编辑

生物技术

Additional file 3: Table S2. Primer sequences used in this article.

DataCite Commons2024-08-16 更新70