A multi-omics workflow to predict the impact of dietary fibers on distinct microbial communities: A simulation study on lean and obese microbial communities

This study investigated the impact of dietary fibers on lean and obese microbial communities, aiming to elucidate the mechanisms underlying their modulation at taxonomical and functional levels. Employing in-vitro digestion and microcolonic fermentations, microbial communities from lean and obese were exposed to an apple matrix, apple pectin (soluble fiber), and cellulose (insoluble fiber). Metagenomics data revealed distinct starting microbial communities and functions in lean and obese individuals. After 24 hours of exposure, diet-responsive bacteria influenced the composition and functionality of microbial communities.