Isolated from a case of pneumonia. Bacillus cereus B4264

Bacillus cereus B4264. This strain was isolated in 1969 from a case of fatal pneumonia in a male patient. B. cereus B4264 was cultured from the blood and the pleural fluid. This isolate was negative for all Laboratory Response Network (LRN) PCR assays that target pXO1 and pXO2. The isolate belongs to MLST type 89 using the MLSTDB scheme. Interestingly, this sequence type appears to be unrelated to B. anthracis. The clinical presentation resembles that of B. cereus G9241 and B. cereus 03BB102, and access to the draft genome of this isolate will allow for the comparison of strains that causes similar disease but have different genetic backgrounds and potential virulence genes. Sequence was obtained from three libraries - a fosmid library (33-39 kb; 50,162 reads), a medium insert library (10-12 kb; 11,376 reads), and a small insert library (3-5 kb; 12,413 reads).