Supplementary_files_Comparative_genomic_analysis_chemosensory-related

The starting datasets were represented by files with the nucleotide sequence corresponding to the genome and proteome of three gastropod species, with their respective annotation files. These data are available in the server of the National Center for Biotechnology Information (NCBI); it was not data generated in our work, as we indicated in materials and methods. Then we used the BITACORA v.1.2.1 software to identify genes from chemosensory families, the output of the analysis yielded files with protein sequences identified by this tool. Alignments were made on these sequences with the Mafft v.7.453 software and phylogenetic trees were built with IQTree v.2.1.2. In addition, we run homemade scripts for the identification of gene clusters by measuring the physical distance among genes. Finally, the genetic distances among genes were estimated with the MEGA-CC v.11.0.11 program.本研究的初始数据集包含三类腹足类（gastropod）物种的基因组与蛋白质组核苷酸序列文件，以及对应的注释文件。上述数据均存储于美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）服务器，并非本研究自主生成，相关细节详见材料与方法部分。随后，本研究使用BITACORA v.1.2.1软件鉴定化学感受基因家族的相关基因，该分析的输出文件包含该工具识别得到的蛋白质序列。使用Mafft v.7.453软件对上述序列开展多序列比对，并通过IQTree v.2.1.2软件构建系统发育树。此外，本研究编写自研脚本，通过计算基因间的物理距离以识别基因簇。最终，借助MEGA-CC v.11.0.11软件估算基因间的遗传距离。