Gene expression from microdissected glomeruli and tubulointerstitium of NZM2328 mice. Gene expression from microdissected glomeruli and tubulointerstitium of NZM2328 mice

To characterize molecular profiles through the development of lupus nephritis and identify risk factors for end organ damage, we carried out gene expression analysis of microdissected kidneys from lupus-prone NZM2328 mice. We identified a continuum of inflammatory processes associated with the progression from acute inflammatory to chronic destructuve disease initiated in the glomeruli and progressing to the tubulointerstitium. We examind male mice and the congenic NZM2328.R27 strain, both of which are resistant to the development of chronic nephritis and end organ damage, as a means to define pathogenic processes. Overall design: RNA was extracted from microdissected kidney glomeruli and tubulointerstitial tissue from female NZM2328 mice with acute (AGN), transitional (TGN), or chronic (CGN) stage glomerulonephritis. NZM2328 male and R27 mice which only develop AGN were also analyzed. For all cohorts, young, non-diseased mice were used as controls. RNA from each sample was then hybridized to Affymetrix arrays for gene expression analysis.