Experimental data for "The developmental mechanics of divergent buckling patterns in the chick gut"

Experimental geometric, modulus, and differential growth raw data, measurments, and MATLAB codes from "The developmental mechanics of divergent buckling patternsin the chick gut": Tissue buckling is an increasingly appreciated mode of morphogenesis in the embryo,but it is often unclear how geometric and material parameters are molecularly determinedin native developmental contexts to generate diverse functional patterns. Here,we study the link between differential mechanical properties and the morphogenesisof distinct anteroposterior compartments in the intestinal tract—the esophagus, smallintestine, and large intestine. These regions originate from a simple, common tubebut adopt unique forms. Using measured data from the developing chick gut coupledwith a minimal theory and simulations of differential growth, we investigate divergentlumen morphologies along the entire early gut and demonstrate that spatiotemporalgeometries, moduli, and growth rates control the segment-specific patterns of mucosalbuckling. Primary buckling into wrinkles, folds, and creases along the gut, as wellas secondary buckling phenomena, including period-doubling in the foregut andmultiscale creasing-wrinkling in the hindgut, are captured and well explained bymechanical models. This study advances our existing knowledge of how identity leads toform in these regions, laying the foundation for future work uncovering the relationshipbetween molecules and mechanics in gut morphological regionalization.