IMOS National Reference Station (NRS) - Zooplankton Abundance

This dataset comprises the zooplankton abundance estimates analysed from samples collected as part of the Integrated Marine Observing Systems (IMOS) National Mooring Network - National Reference Station (NRS) field sampling. Regular monthly zooplankton samples are undertaken at the stations as part of a larger water sampling program by the Australian National Reference Stations. In total nine stations are sampled regularly around the Australian coastline. Sampling is conducted monthly (Esperance and Ningaloo 3 monthly) off small vessels at each of the nine reference stations. The sampling is conducted using a drop net (as per the design of Heron) which has a 60 cm diameter, 100 micron mesh and is weighted to fall at 1 m/s. The net is designed to pull closed at the end of its fall so that it samples on the way down and does not sample on the way up. The depth of the sample varies at each station. 3 zooplankton samples are taken each month. 2 are fixed in formalin and will be analysed as described below. The unfixed sample is concentrated and frozen (-80oC)and will be used for molecular analysis in the future. The Port Hacking 4 (PH4) samples (2002 - Jan 2009) were sampled using a smaller net, 20 cm diameter, which sampled on the way up and down. The PH4 site is very close to the Port Hacking national reference station (PHB) where samples are now taken to build up and enhance the time series. The plankton ecology lab (based in Queensland) will analyse the samples collected for: 1. Community composition (Taxon/m3) 2. Biomass (dry weight in mg/m3) 3. Size spectrum analysis using zooscan (in progress) Data storage and access is planned to be interoperable with other national and international programs through the IMOS Infrastructure. Station metadata can be found through this resource. Data is available freely via the AODN portal: https://portal.aodn.org.au. As the taxonomic resolution of the data has changed over time, due to continual training, it is important that users refer to the change log tables included in your data download. These will provide information on the validity of the taxa, from what date we have been identifing certain taxa etc. Classification fields may be blank depending on the level to which that taxa has been identified, i.e. if only identified to family, genus and species will be blank. Additional information for this dataset may be available via the original MarLIN metadata entry: https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/c13451a9-7cfe-091c-e044-00144f7bc0f4. For information on using the data please refer to Eriksen et al. 2019. It is advised that anyone using this data should read this methodology or contact the project contact person. The data are made available in binned products for ease of use. As taxonomy changes over time and the ability of analysts improves with training more and more species are able to be identified over time. The products account for these changes and for real absences from the samples. A 0 abundance means that the species was looked for and not seen, -999 means that the species was not looked for in that sample. The products are therefore applicable for use in time series analysis. There are 5 products to choose from: Raw product (includes all data); Copepod species file (includes all copepod data where species have been identified); Non copepod species file (includes all non copepod data where species have been identified); Genus product (includes all data binned to genus level); Higher taxonomic group (includes all data binned to functional groups). The binned and pivoted products described are currently only available by contacting [e-mail]. A project to make these products available directly via the portal is under way. This collection currently only provides direct access to the raw data in "flat table" form (separate row for each taxon in each sample). **This dataset has been superseded by the "IMOS - Zooplankton Abundance and Biomass (reference stations)" collection that is available at https://catalogue-imos.aodn.org.au/geonetwork/srv/api/records/bc14672f-c007-408e-972b-684d8415dae8 (this collection was removed from the AODN Portal in December 2021).**

本数据集涵盖了从综合海洋观测系统（Integrated Marine Observing Systems, IMOS）国家锚定网络-国家参考站（National Reference Station, NRS）野外采样所获取的样本中分析得到的浮游动物丰度估算值。
澳大利亚国家参考站作为大型水体采样计划的一部分，定期在各站点开展月度浮游动物采样。目前澳大利亚沿海岸线共设有9个常规采样参考站，采样工作由小型船舶在各站点每月开展一次（埃斯佩兰斯和宁格罗地区为每3个月一次）。
采样采用赫伦（Heron）设计的沉降式网具，网口直径60cm，网目尺寸100微米，配重设置使其沉降速度为1m/s。该网具设计为在沉降结束时自动闭合，仅在下沉过程中完成采样，上升过程不进行采样。各站点的采样深度存在差异。
每月采集3份浮游动物样本，其中2份用福尔马林固定，将按照下述方法进行分析；剩余1份未固定样本经浓缩后冻存于-80℃环境，将用于未来的分子生物学分析。
哈克宁港4号站点（Port Hacking 4, PH4）的样本（2002年1月至2009年）采用直径20cm的小型网具，在下沉和上升过程中均进行采样。PH4站点距离当前开展采样以完善时间序列的哈克宁港国家参考站（PHB）极近。
位于昆士兰州的浮游生物生态实验室将对采集的样本开展以下分析：1. 群落组成（分类单元/立方米）；2. 生物量（干重，单位mg/m³）；3. 利用浮游生物扫描系统（Zooscan）开展的粒径谱分析（进行中）。
本数据集计划通过IMOS基础设施实现与其他国家及国际项目的互操作。站点元数据可通过本资源获取，数据可通过澳大利亚海洋数据网络（Australian Ocean Data Network, AODN）门户免费获取：https://portal.aodn.org.au。
由于持续的分类学培训优化，本数据集的分类学分辨率随时间推移有所变化，因此用户务必参考数据下载包中附带的变更日志表格，该表格将提供分类单元的有效性信息、特定分类单元的识别起始日期等内容。分类字段可能留空，具体取决于该类群的鉴定水平：例如，若仅鉴定至科水平，则属和种字段将为空。
本数据集的更多信息可通过原始MarLIN元数据条目获取：https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/c13451a9-7cfe-091c-e044-00144f7bc0f4。关于数据使用方法，请参考Eriksen等人2019年的研究，建议所有使用本数据的人员阅读该方法文档或联系项目联系人。
本数据集以分箱产物形式发布，便于用户使用。随着分类学方法的迭代与分析人员能力的提升，可识别的物种数量随时间不断增加。这些分箱产物兼顾了上述变化以及样本中真实的物种缺失情况：丰度值为0代表该物种在该样本中被检出但未发现个体，值为-999则代表该物种未在该样本中开展检测。因此，这些产物适用于时间序列分析。
本次可供选择的分箱产物共5类：1. 原始产物（包含全部数据）；2. 桡足类物种文件（包含所有已完成物种鉴定的桡足类数据）；3. 非桡足类物种文件（包含所有已完成物种鉴定的非桡足类数据）；4. 属级产物（包含所有归类至属级的数据）；5. 高级分类群产物（包含所有归类至功能群的数据）。
当前上述分箱及枢轴化产物仅可通过联系[e-mail]获取，一项可通过门户直接提供这些产物的项目正在推进中。本数据集集合目前仅提供"扁平表"形式的原始数据直接访问权限（每个样本中的每个分类单元对应单独一行）。
**本数据集已被现有的"IMOS-浮游动物丰度与生物量（国家参考站）"数据集集合取代，该新数据集可通过https://catalogue-imos.aodn.org.au/geonetwork/srv/api/records/bc14672f-c007-408e-972b-684d8415dae8获取（本数据集已于2021年12月从AODN门户下架）。**